Application

Western Blotting at 5-10 µg/mL: recognizes a band of approximately 28-30kDa in reduced samples.

Immunohistochemistry: frozen and 4%PFA fixed paraffin sections: 5-20µg/mL enzymatic detection.



Optimal working dilutions must be determined by end user.

Research CategoryCell Structure

Anti-TIMP-1 Antibody detects level of TIMP-1 & has been published & validated for use in ELISA, WB, IH.

Research Sub CategoryMMPs & TIMPs

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Full length purified human fibroblast tissue inhibitor of metalloproteinases {Clark, IM (1991) Matrix 11:76-85}.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1133

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin. Liquid in PBS containing 0.02% azide.

Specificity

Reacts with TIMP1. Does not react with any other human proteins tested so far. The antibody reacts with native and denatured TIMP1 and TIMP1 in and out of enzyme/inhibitor complex.

Storage and Stability

Maintain refrigerated at 2-8°C for up to three months from date of receipt. For extended storage, freeze in convenient undiluted aliquots at -20°C for up to twelve months from date of receipt. Avoid repeated freeze/thaw cycles.